Fig. 4.

ARMS analysis of Cimicifuga plants in trnL−trnF IGS region. Data taken from reference 20. a PCR amplification using a specific primer set for C. racemosa. b PCR amplification using a primer set for other Cimicifuga species. The sequences of a species-specific sense primer for C. racemosa and another five Cimicifuga species: 5′-CCTGAGCCAAATCCTGTTCTA-3′ and 5′-CCTGAGCCAAATCCTGTTCTC-3′. The sequence of a common anti-sense primer: 5′-TCAAATCAGCCACTCCATAGTATGA-3′. Sample IDs are shown in Table 2. M 100 bp DNA ladder