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. 2016 Feb 24;310(11):F1251–F1257. doi: 10.1152/ajprenal.00024.2016

Fig. 5.

Fig. 5.

miR-466g decreases aldosterone-induced increase in SGK1 expression in mpkCCDc14 cells. Polarized mpkCCDc14 cells, transduced with miR-466g or nontargeting siRNA, were treated with aldosterone (10−6 M; Aldo) or vehicle (Veh) for 1, 6, or 24 h. Expression of SGK1 mRNA was measured by quantitative PCR and normalized to expression of GAPDH. Relative expression of SGK1 mRNA in cells transduced with miR-466g (OE-miR-466g) or GAPDH (OE-GAP) treated with aldosterone is shown as fold change over SGK1 mRNA in corresponding cells treated with vehicle. Values are means ± SE. *P < 0.05 between cells transduced with GAPDH and treated with aldosterone (OE-GAP Aldo) and cells transduced with miR-466g and treated with aldosterone (OE-miR-466g Aldo) at the 6-h time point. Results are from 7 independent experiments from cells of different passages.