Fig. 1.
Glutamine synthetase (GS) expression in GSfl/fl, phosphoenolpyruvate carboxykinase (PEPCK)-Cre-negative and GSfl/fl, PEPCK-Cre-positive mice. Top: representative low-power micrographs of GS expression in control mouse kidneys and kidneys from mice with proximal tubule (PT)-specific GS deletion (PT-GS-KO). A clear decrease in GS immunoreactivity was evident in PEPCK-Cre-positive kidneys. Results are representative of findings of n = 6 mice/genotype. Bottom: immunoblot analysis of GS expression in the cortex and outer stripe of the outer medulla (OSOM) of control and PT-GS-KO mice. PT-GS-KO resulted in significant decreases in GS expression in both the cortex and OSOM. Values are means ± SE; n = 8 mice/genotype.