Fig. 2.
Effect of aliskiren on prorenin-induced Na+ transport in cultured CD cells. Confluent mpkCCD cells grown on Transwells were pretreated for 30 min with aliskiren (1.0 μM) and then treated with prorenin (10 nM). Ieq was and amiloride-sensitive Ieq were determined as described in Fig. 1. A: time course of Ieq changes in control, prorenin, and prorenin + aliskiren groups. Prorenin was added to the apical side. B: corresponding amiloride-sensitive Ieq for experiments in A. C: comparison of the effect of apical and basal administration of prorenin on Ieq. Data are means ± SE; n = 6–12 per group. *P < 0.05 vs. basal in the same group.