Fig. 3.

Effect of renin on ENaC activity in cultured CD cells. Confluent mpkCCD cells grown on a Transwell membrane were pretreated for 30 min with PRO20 (1.5 μM) and then treated with renin (10 nM). A: time course of I_eq changes in Control, renin, and renin + PRO20 groups. I_eq was monitored for 15 min. Renin was given at 10 nM. B: time course of I_eq changes in control and renin groups was monitored for 15 min. Renin was given at 100 nM. C: amiloride-sensitive I_eq in control, renin, and renin + PRO20 groups. Data are means ± SE; n = 6–12 per group. *P < 0.05 vs. basal in the same group.