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. 2016 Jul 6;7:12119. doi: 10.1038/ncomms12119

Figure 5. Morphological phenotypes and biochemical characterization of hpGFT1 lines.

Figure 5

(a) Representative phenotypes (cohorts) of plants transformed with a hairpin construct specifically targeting GFT1 expression compared with control plants (plants transformed with the empty vector). Resultant plants were assigned to four categories based on the severity of the phenotype. Scale bar, 10 mm (b) Total L-fucose content from cell wall extracts (Fucose) derived from hpGFT1 lines and the corresponding reductions in GFT1 expression in these lines. GONST3 expression was not significantly affected in the hpGFT1 lines. The control values are mean and s.d. of three independent measurements (n=3). The values for the hpGFT1 lines are the mean of each cohort analysed in triplicate. The ‘average' is the mean and s.d. (n=4) of the data from the four independent hpGFT1 cohorts. Fucose content (P<0.003) and GFT1 transcript levels (P<0.005) are significantly different by Student's t-test. Values are expressed relative to control lines (plants transformed with the empty vector).