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. 2016 Jul 6;7:12102. doi: 10.1038/ncomms12102

Figure 1. Compartment-specific roles of K+ channels in action potential shape in cerebellar basket cells.

Figure 1

(a) Representative action potentials recorded from the soma (left) or bouton (right) of cerebellar basket cells, before (black trace) and after (green trace) Kv1.1 blockade with DTx-K. Top: schematics illustrating recording sites. Inset: action potentials at slow time base, showing a prominent ADP at the bouton. Scale bar, 20 mV; 2 ms (main traces); 40 mV, 200 ms (insets). The fluorescence image at right shows a cerebellar basket cell bouton labelled with Alexa 568. G and P indicate granule cell and Purkinje cell layer, respectively. Scale bar, 10 μm. (b) Summary data showing selective effect of DTx-K on presynaptic spike width measured at –30 mV. Circles show individual experiments. (c) BKCa blockade with ChTx led to somatic action potential broadening. *P<0.05, ***P<0.001; paired t-tests.