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. 2016 Jul 7;6:29211. doi: 10.1038/srep29211

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Copyright © 2016, Macmillan Publishers Limited

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(A) The light microscope results for M. incognita processed with the P. putida 1A00316 protein. ‘a’, ‘b’ and ‘c’ processed with the nematicidal protein for 0 h, 12 h and 24 h respectively. (B) The purified fraction exhibiting nematicidal activity after Sephadex G75 chromatography was assayed by SDS-PAGE. M represents the protein marker, line 1 represents the P. putida 1A00316 purified fraction exhibiting nematicidal activity after Sephadex G75 chromatography. (C) Protein bands detected by MALDI-TOF MS. The six red amino acid fragments were peptides, and can all be matched to a protein annotated alkaline metalloproteinase AprA. (D) aprA gene cluster in different Pseudomonas strains.