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. 2016 Jul 7;6:29226. doi: 10.1038/srep29226

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The hph cassette was amplified from pBGgHg and has the Agaricus bisporus gpdII promoter driving hph with the CaMV 35S terminator³⁰. Armillaria mellea (Am) long and short introns are introns 11 and 7, respectively, from putative efflux transporter genes (NCBI reference EF547153 and EF547152, respectively)⁴⁸. The Phanerochaete chrysosporium gpd (Pc gpd) promoter, P. chrysosporium intron/exon, eGFP and the Aspergillus nidulans trpC terminator (An trpC) were amplified from pGR4-GFP or pGR4-4iGM3²³. mRFP was amplified from pYES-hph-RFP004²⁴. The sequence of the A. mellea gpd promoter (Am gpd) and intron was based on the gene model with protein ID 13125¹³.