Table 1. Armillaria mellea and Clitopilus passeckerianus transformants obtained with various vectors and the percentage showing expression of GFP or mRFP.
| Vector | Promoter | Intron | Number of C. passeckerianus transformants (% expressing GFP/mRFP) | Number of A. mellea transformants (% expressing GFP/mRFP) |
|---|---|---|---|---|
| pBGgHg30 | Agaricus bisporus gpdII | — | 29 (0%) | 31 (0%) |
| pGR4-4iGM323 | A. bisporus gpdII | P. chrysosporium gpd | n/a | 27 (0%) |
| pGR4-GFP23 | Phanerochaete chrysosporium gpd | P. chrysosporium gpd | n/a | 23 (0%) |
| pCAM-hph-GFP | P. chrysosporium gpd | — | 6 (0%) | 10 (0%) |
| pCAM-hph-mRFP | P. chrysosporium gpd | — | 11 (0%) | 23 (0%) |
| pCAM-hph-Amgpd-GFP | Armillaria mellea gpd | — | 5 (0%) | 10 (0%) |
| pCAM-hph-Amgpd-mRFP | A. mellea gpd | — | 13 (0%) | 11 (0%) |
| pCAM-hph-LiGFP | P. chrysosporium gpd | A. mellea eta | 6 (0%) | 10 (0%) |
| pCAM-hph-siGFP | P. chrysosporium gpd | A. mellea etb | 4 (100%) | 17 (0%) |
| pCAM-hph-simRFP | P. chrysosporium gpd | A. mellea etb,† | 6 (0%) | 11 (0%) |
| pCAM-hph-iGFP | P. chrysosporium gpd | A. mellea gpd | 5 (40%) | 10 (0%) |
| pCAM-hph-imRFP | P. chrysosporium gpd | A. mellea gpd | 3 (33%) | 16 (0%) |
| pCAM-hph-xiGFP | P. chrysosporium gpd | P. chrysosporium gpd | 26 (96%) | 18 (0%) |
| pCAM-hph-Amgpd-iGFP | A. mellea gpd | A. mellea gpd | 4 (100%) | 10 (20%) |
| pCAM-hph-Amgpd-imRFP | A. mellea gpd | A. mellea gpd | 1 (100%) | 4 (75%) |
| pCAM-hph-Amgpd-xiGFP | A. mellea gpd | P. chrysosporium gpd | 5 (60%) | 10 (30%) |
| pCAM-hph-Amgpd-ximRFP | A. mellea gpd | P. chrysosporium gpd | 4 (75%) | 6 (50%) |
All vectors constructed contain the hph cassette from pBGgHg, where hygromycin phosphotransferase is under regulatory control of the Agaricus bisporus gpdII promoter and CaMV 35S terminator. Vectors contain either eGFP and mRFP (suffixed GM3/GFP and mRFP, accordingly) and are under regulatory control of either the Phanerochaete chrysosporium gpd promoter or Armillaria mellea gpd promoter (as indicated) with the Aspergillus nidulans trpC terminator.
aContains intron 11 from a putative A. mellea efflux transporter (EF547153).
bContains intron 7 from a putative A. mellea efflux transporter (EF547152).
†Vector pCAM-hph-simRFP has a 2 bp (TT) deletion in the intron sequence at positions 12 and 13.