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. 2016 Jul 7;6:29041. doi: 10.1038/srep29041

Figure 2. TRPC6 channel activation inhibits proliferation and promotes apoptosis of neonatal GMCs.

Figure 2

(a) Phase contrast images (segmentation mask illustrated in goldenrod; HF, 10 μM) and (b) Cell growth curves showing time- and concentration-dependent anti-proliferative effect of HF (n = 4 each) in neonatal pig GMCs. (c) Images (green fluorescent staining of nuclear DNA in apoptotic cells; HF, 10 μM) and (d) Kinetic curves demonstrating that HF (n = 4 each) induces time- and concentration-dependent increase in caspase-3/7 activity in neonatal pig GMCs. (e) Images and (f) Kinetic curves illustrating that TRPC6 channel knockdown attenuates HF (10 μM; n = 4 each)-induced caspase-3/7 activity in neonatal pig GMCs. #P < 0.05 vs. control (DMSO; 12–36 hr); *P < 0.05 vs. control (DMSO; 10–36 hr); $P < 0.05 vs. control (DMSO; 6–36 hr); **P < 0.05 vs. control (DMSO; 10–36 hr); ***P < 0.05 vs. control (DMSO; 2–36 hr); %P < 0.05 vs. control siRNA (20–36 hr); Scale bars = 100 μm.