Figure 6. TRPC6 channel-dependent [Ca²⁺]_i elevation and successive activation of the NFAT and FasL/Fas signaling pathways trigger neonatal GMC apoptosis.

(a) Representative microplate graphs and (b) Kinetic curves showing HF-induced caspase-3/7 activity in control and BAPTA (1 μM; n = 10)-, FK506 (100 nM; n = 10)-, VIVIT (1 μM; n = 10)-, Ac-DEVD-CHO (50 μM; n = 10)-, and Fas BA (10 μg/mL; n = 5)-treated GMCs. Cells were pretreated with blockers/inhibitors for ~30 min before the addition of HF. *P < 0.05 vs. control (8–36 hr); ^#P < 0.05 vs. control (10–36 hr).