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. 2016 Jul 7;6:29139. doi: 10.1038/srep29139

Table 1. Plasmids used in this study.

Plasmid name Description Source
hCOG1 1-702-3Myc Human COG1(aa1-702) in pCMV-3Myc This study
hCOG1-3Myc Human Cog1siRES in pCMV-3Myc Willett et al.6
mCOG3-3Myc Mouse COG3 in pCMV-3Myc Willett et al.6
hCOG7-3Myc Human COG7 in pCMV-3Myc Ha et al.15
hCOG6-3Myc/hCOG8-3Myc Human COG6-3Myc and COG8-3Myc in MultiLabel vectors (ATG:biosynthetics GmbH) Willett et al.6
hCOG5-3Myc/hCOG7-3Myc Human COG5-3Myc and COG7-3Myc in MultiLabel vectors (ATG:biosynthetics GmbH) Willett et al.6
hCOG2-Strep-3Myc/hCOG4-3Myc/hCOG1-3Myc Human COG1-3Myc, COG2Streptag-3Myc, and COG4-3Myc in MultiLabel vectors (ATG:biosynthetics GmbH) Willett et al.6
hCOG1-(HA-GS-) or (GFP-GS-) TEV-hCOG8-3Myc Human COG1-siRES, COG8-siRES and TEV protease recognition sequence in pCMV-3Myc This study
hCOG1-TEV-3Myc Human COG1-siRES and TEV protease recognition sequence in pCMV-3Myc This study
hCOG8sir-mCh-ActA Human COG8siRES in pmCherry-ActA Willett et al.22
hCOG8sir-GFP-ActA Human COG8siRES in pGFP-ActA This study
hCOG8sir 1–406 GFP-ActA Human COG8siRES (aa1–406) in pGFP-ActA This study
COG8sir 1–136 GFP-ActA Human COG8siRES (aa1–136) in pGFP-ActA This study
hCOG8sir Δ8–406 GFP-ActA Human COG8siRES (aaΔ8–406) in pGFP-ActA This study
hCOG8sir Δ8-184-3Myc Human COG8siRES (aaΔ8-184) in pCMV-3Myc Willett et al.22
GFP-hGS15 Human GS15 in pEGFP-C1 Shestakova et al.17
GFP-P115 P115 in pEGFP-C2 E. Sztul
GFP-ActA   A. Linstedt
GFP-hCOG6 Human COG6 in pGFP-C1 Pokrovskaya et al.9
GFP-mCOG3 Mouse COG3 in pEGFP-C1 Zolov et al.40
GFP-hCOG5 Human COG5 in pEGFP-C1 Ha et al.15
GFP pEGFP-C1  
His-hGS15-GST Human GS15 cytoplasmic domain (aa 1–86) with N-terminal polyhistidine tag and C-terminal GST tag in pET28a This study
GST pGEX4T-1