Table 2.

Prioritized mutations from next generation sequencing of STICs and synchronous gynecologic malignancies

Patient	Site	Gene	Exonic Function	Location	Nucleotide	Amino Acid	Variant Allele Frequency	Coverage at Variant Allele
1	Tube	BRCA2	frameshift deletion	chr13:32914209	c.5718_5719del	p.1906_1907del	72%	137
	Tube	TP53	stopgain SNV	chr17:7578263	c.C190T	p.R64X	48%	85
	Ovary	BRCA2	stopgain SNV	chr13:32972626	c.A9976T	p.K3326X	50%	87
	Ovary	BRCA2	frameshift deletion	chr13:32914209	c.5718_5719del	p.1906_1907del	54%	122
	Ovary	TP53	stopgain SNV	chr17:7578263	c.C190T	p.R64X	11%	8
2	Tube	BRCA1	stopgain SNV	chr17:41244787	c.C2620T	p.Q874X	52%	464
	Tube	TP53	nonsynon. SNV	chr17:7577580	c.A305G	p.Y102C	10%	118
	Ovary	TP53	nonsynon. SNV	chr17:7577580	c.A305G	p.Y102C	69%	225
	Ovary	BRCA1	stopgain SNV	chr17:41244787	c.C2620T	p.Q874X	88%	351
3	Tube	TP53	nonsynon. SNV	chr17:7577535	c.G350C	p.R117T	15%	90
	Uterus	MTOR	nonsynon. SNV	chr1:11184573	c.C6644A	p.S2215Y	18%	36
	Uterus	PTEN	nonsynon. SNV	chr10:89685308	c.A203T	p.Y68F	15%	41
	Uterus	KRAS	nonsynon. SNV	chr12:25398284	c.G35T	p.G12V	20%	81
	Uterus	PIK3CA	nonsynon. SNV	chr3:178916876	c.G263A	p.R88Q	21%	85
	Uterus	ATM	nonsynon. SNV	chr11:108142000	c.C2944T	p.R982C	20%	81
4	Tube	TP53	nonsynon. SNV	chr17:7577548	c.G337C	p.G113R	12%	60
	Tube	PTEN	stopgain SNV	chr10:89692904	c.C388T	p.R130X	18%	36
	Tube	CTNNB1	nonsynon. SNV	chr3:41266137	c.C134T	p.S45F	14%	151
	Uterus	PTEN	stopgain SNV	chr10:89692904	c.C388T	p.R130X	34%	73
	Uterus	CTNNB1	nonsynon. SNV	chr3:41266136	c.T133C	p.S45P	33%	133

nonsynon. SNV = nonsynonmous single nucleotide variant.