Figure 2. Impact of doxycycline on the AUC of RT-QuIC seeding response of sCJD brain and CSF samples.
The AUC was defined as the parameter of interest to characterise the RT-QuIC seeding response (A) RT-QuIC reactions, seeded with brain tissue in a dilution of 10−3 from sCJD (MM1) patients (n = 5) were treated with different concentrations (0.0, 0.1, 0.3, 0.5 and 1.0 mM) of doxycycline. Doxycycline concentrations of 0.1 mM or higher resulted in an inhibition of the seeding of PrP. (B) The inhibition of the PrP conversion via doxycycline was also obtained in RT-QuIC reactions seeded with CSF from sCJD (MM1) patients (n = 14). Doxycycline concentrations of 0.5 mM or higher resulted in an inhibition of the seeding of PrP. Non-treated reactions were used as a reference (0.0). A p-value: <0.001 as extremely significant (***), <0.01 as very significant (**), <0.05 as significant (*) and ≥0.05 as not significant (ns).