Table 1. Incubation time of tg338 mice inoculated with serial tenfold dilutions of Cell-based mb-PMCA-generated 127S prions.
| Dilution | Incubation time in days ± SEM (n/n0) | ||
|---|---|---|---|
| PMCA substrate | |||
| Cell lysate − DSS | Cell lysate + DSS | tg338 brain | |
| 10−1 | 75 ± 1 (5/5) | 93 ± 1 (5/5) | 60 ± 1 (4/4) |
| 10−3 | 91 ± 2 (5/5) | 100 ± 4 (5/5) | 72 ± 2 (5/5) |
| 10−4 | 108 ± 6 (5/5) | ND | |
| 10−5 | 114 (1/5)* | 98; 124 (2/5)* | 98 ± 3 (5/5) |
| 10−6 | >240 (0/5)* | ND | |
| 10−7 | >240 (0/5)* | >240 (0/5)* | 124 (1/5) |
| Unseeded | >240 (0/5)* | >240 (0/5)* | |
Amplicons obtained from a 10−7 127S seed mixed with 10 mg/mL cell lysates supplemented with PrP0/0 brain, in the absence (−DSS) or presence (+DSS) of 1% dextran sulfate, were tenfold diluted up to the 10−7 dilution and immediately inoculated intracerebrally to reporter tg338 mice. n/n0: number of mice with neurological disease and positive for PrPres in the brain by immunoblotting/number of inoculated tg338 mice.
*Non-affected mice euthanized healthy at 240 dpi. Data in italic are from22. ND: not done.