Figure 3.

Effect of methylation on obp11 alternative splicing. (a) Alternative splice acceptor site in intron 3 generates transcripts with a premature stop codon resulting in truncated peptide. Primers used to examine the expression of full-length (FL) and truncated (T) transcripts are shown below the exon/intron map. (b) Expression of obp11 alternatively spliced variant shown as percentage of total obp11 expression. In drone antennae, the spliced variant with the premature stop codon constitutes a much higher proportion of obp11 transcripts. This result is based on five replicates. (c) Increased methylation of three CpG sites in exon 3 correlates with higher abundance of the spliced variant encoding the truncated obp11. Based on three libraries. **p < 0.01, Student's t-test. (Online version in colour.)