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. 2016 Jun 29;36(14):1931–1942. doi: 10.1128/MCB.00118-16

FIG 5.

FIG 5

The effects of AICAR and t-BHQ on the activation of Nrf2. (A) Functional network between AMPK and Nrf2. The network was obtained from an open database (HumanNet). A dotted box captures the association of PKCδ and AMPK or Nrf2. (B) Immunoblottings for Nrf2 in HepG2 cells treated with AICAR and/or 30 μM t-BHQ for 3 h. WCL, whole-cell lysates; NF, nuclear fraction; CF, cytoplasmic fraction. (C) NQO1-ARE reporter assays and qRT-PCR assays for HMOX1 in HepG2 cells treated with AICAR and/or t-BHQ for 18 h. (D) Immunoblottings for p-AMPKα in HepG2 cells treated with AICAR or AICAR plus t-BHQ for 30 min. (E) NQO1-ARE reporter assays. HEK293 cells were treated with either t-BHQ or AICAR for 6 h after cotransfection with Myc-tagged WT-Nrf2 or S550A-Nrf2 for 24 h. For panels C and E, data represent means ± SEM of the results from three different cell preparations (significantly different from vehicle control results [**, P < 0.01; *, P < 0.05] and from AICAR-treated control results [##, P < 0.01]; N.S., not significant).