FIG 2.

Four miRNAs predicted in silico to target the NPPA 3′ UTR reduce the luciferase activity produced by the firefly luciferase-NPPA 3′ UTR (WT-Luc) construct. The ratio of firefly luciferase activity to renilla luciferase activity was normalized to the ratio in COS-7 cells transfected with constructs encoding luciferase without the NPPA 3′ UTR and renilla. The effect of each miRNA was compared to that of a scrambled negative-control miRNA mimic (relative luciferase activity). miR-155 (A), miR-103 (B), miR-107 (C), and miR-105 (D) reduced the activity of the WT-Luc construct. Data are means ± SEMs (n = 6 biological replicates; two-tailed independent t test).