FIG 6.

miR-105 interacts with the NPPA 3′ UTR in an rs61764044 allele-specific manner. (A) miR-105 reduced the activity of the WT-Luc construct but not the rs5067 Minor-Luc construct. The ratio of firefly luciferase activity to renilla luciferase activity was normalized to the ratio in COS-7 cells transfected with constructs encoding luciferase without the NPPA 3′ UTR and renilla. For each construct (WT-Luc or rs5067 Minor-Luc), the effect of the miRNA was compared to that of a scrambled negative-control miRNA mimic (relative luciferase activity). (B) Anti-miR-105 increased the activity of the WT-Luc construct but not rs5067 Minor-Luc construct. The ratio of firefly luciferase activity to renilla luciferase activity was normalized to the ratio in COS-7 cells transfected with constructs encoding luciferase without the NPPA 3′ UTR and renilla. For each construct (WT-Luc or rs5067 Minor-Luc), the effect of the anti-miRNA was compared to that of a scrambled negative-control anti-miRNA (relative luciferase activity). Data are means ± SEMs (n = 6 biological replicates; two-tailed independent t test).