FIG 7.
Anti-miR-105 increases NPPA mRNA and secreted Nt-proANP protein levels in human cardiomyocytes. hESC-CMs (∼1 × 105 per well) were transfected with miR-105, a scrambled negative-control miRNA mimic, anti-miR-105, or a scrambled negative-control anti-miRNA. Twenty-four hours later, cells were washed and incubated in 1 ml of medium. After an additional 24 h, cells and medium were harvested. (A) NPPA mRNA levels in hESC-CMs transfected with miR-105 relative to negative-control miRNA mimic. (B) Nt-proANP protein levels in the medium of hESC-CMs transfected with miR-105 relative to negative-control miRNA mimic. (C) NPPA mRNA levels in hESC-CMs transfected with anti-miR-105 relative to negative-control anti-miRNA. (D) Nt-proANP protein levels in the medium of hESC-CMs transfected with anti-miR-105 relative to negative-control anti-miRNA. Data are means ± SEMs (n = 6 biological replicates; two-tailed independent t test).