FIG 1.

Generation of aP2 and Adn-AMPKα1/α2 ASKO mice. (A) RT-qPCR for AMPKα1 and AMPKα2 expression in the SVFs and adipocytes of wild-type mouse WAT (n = 4). (B) Cre-Lox strategy for removal of AMPKα1 (Prkaa1) and -α2 (Prkaa2) from adipose tissue. Open boxes, untranslated regions; gray boxes, translated regions that do not encode the kinase domain; black boxes, translated regions that encode the respective kinase domain; triangles, loxP sites; arrows, primer sets for genotyping (top). RT-qPCR for AMPKα1 (left) and AMPKα2 (right) expression in various tissues from aP2-ASKO (a) and Adn-ASKO (b) mice and control Flox/Flox mice (n = 4). (C) Immunoblotting and its quantification of lysates from various adipose depots, gonadal (Gon), inguinal (Ing), renal (Reno), and liver, from aP2-ASKO (a) and Adn-ASKO (b) mice with AMPKα, AMPKα1, and AMPKα2 antibodies. GAPDH was used as an internal control (n = 4). Data are expressed as means ± SEM. *, P < 0.05; **, P < 0.01; NS, no significant change. Experiments were repeated twice, and representative data are shown.