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. 2016 Jun 29;36(14):1961–1976. doi: 10.1128/MCB.00244-16

FIG 2.

FIG 2

AMPK phosphorylates desnutrin/ATGL and HSL. (A) Immunoblotting and its quantification of WAT lysates with antibodies against phosphorylated desnutrin/ATGL (P-S406) and total desnutrin/ATGL in aP2-ASKO (a) and Adn-ASKO (b) mice. WAT were harvested under fasted conditions (9 a.m.). (B) Immunoblotting and its quantification of WAT lysates with antibodies against HSL and phospho-HSL (P-S565, P-S660, and P-S563) in aP2-ASKO (a) and Adn-ASKO (b) mice (n = 4). (C and D) Immunoblotting and its quantification of desnutrin/ATGL and its phosphorylation (C) and HSL and its phosphorylation (D) in WAT lysates under basal or isoproterenol (Iso)-stimulated conditions (n = 4). Experiments were repeated twice, and a representative blot is shown. *, P < 0.05; **, P < 0.01.