FIG 5.

AMPK-ASKO mice show a lean phenotype. (A) BW gain, food intake, lean mass, and fat mass gain of aP2-ASKO (a) and Adn-ASKO (b) mice (n = 8) fed a standard chow diet. (B) Fat pad and liver weights of aP2-ASKO (a) and Adn-ASKO (b, right side) mice (n = 8). Data from male Adn-ASKO mice on a chow diet at 16 weeks of age (b, left side) and gonadal (Gon), inguinal (Ing), renal (Reno), and liver samples from these mice (b, middle) are also shown. (C) Hematoxylin-and-eosin staining of gonadal fat pads (left) and quantification of cell size (right, n = 3). Scale bar = 20 μm. (D) TAG contents (left), relative levels of DAG (middle), and FFA levels (right). The levels in Flox/Flox mice were defined as 100% (middle and right, n = 4). (E) Serum FFA levels in aP2-ASKO (a) and Adn-ASKO (b) mice (n = 4). (F, a) RT-qPCR for lipogenic gene expression in WAT (n = 4). (F, b) Immunoblotting of WAT lysates with antibodies to ACC, phosphorylated ACC (P-S79ACC), and AMPKα. GAPDH was used as an internal control. (F, c) Lipids generated were measured by counting [¹⁴C]TAG after incubation of gonadal WAT fragments (with or without treatment with insulin at 1 μg/ml for 15 min) with d-[6-¹⁴C]glucose for 2 h. Lipids were separated by TLC. Experiments were repeated twice, and representative data are shown. Data are expressed as means ± SEM. *, P < 0.05; **, P < 0.01; NS, no significant change.