FIG 6.

AMPKα1/α2 ablation promotes FA oxidation in adipose tissue. (A) O₂ consumption rate (VO₂) determined by indirect calorimetry in 16-week-old mice on a chow diet (left and middle; n = 6). The RER is shown on the right. (B) FA oxidation was measured by determining captured CO₂ and ASMs after the incubation of lysates with [¹⁴C]palmitate for aP2-ASKO (a) and Adn-ASKO (b) mouse WAT, Adn-ASKO mouse liver (c), and Adn-ASKO mouse muscle (d). (n = 4). (C) RT-qPCR for FA oxidative genes in aP2-ASKO (a) and Adn-ASKO (b) mouse WAT and aP2-ASKO (c) and Adn-ASKO (d) mouse liver (n = 4). Data are expressed as means ± SEM. *, P < 0.05; **, P < 0.01; NS, no significant change.