TABLE 1.
Bacterial strains used in this study
| Bacterial species and strain | Strain designationa | Relevant genotype or other informationb | Reference or source |
|---|---|---|---|
| S. aureus NRS384 | WT | ΔhsdR ΔsauUSI | 14 |
| B. subtilis 1A771 | EK1 | amyE::pDG1661-B. subtilis xpt(−305)-lacZ | This workc |
| B. subtilis 1A771 | EK2 | amyE::pDG1661-S. aureus xpt(−452)-lacZ | This work |
| S. aureus NRS384 | EK3 | ΔhsdR ΔsauUSI ϕ85att::pIMC85-S. aureus xpt(−452)-lacZ | This work |
| S. aureus NRS384 | EK4 | ΔhsdR ΔsauUSI ϕ85att::pIMC85-S. aureus pbuX(−568)-lacZ | This work |
| S. aureus NRS384 | EK5 | ΔhsdR ΔsauUSI ϕ85att::pIMC85-S. aureus guaB(−112)-lacZ | This work |
| S. aureus NRS384 | EK6 | ΔhsdR ΔsauUSI ϕ85att::pIMC85-S. aureus guaA(−414)-lacZ | This work |
| S. aureus NRS384 | EK7 | ΔhsdR ΔsauUSI ϕ85att::pIMC85-S. aureus guaA(−764)-lacZ | This work |
| S. aureus NRS384 | EK8 | ΔhsdR ΔsauUSI Δxpt-pbuX | This work |
| S. aureus NRS384 | EK9 | ΔhsdR ΔsauUSI pbuX::TnERM | This work |
| S. aureus NRS384 | EK10 | ΔhsdR ΔsauUSI Δxpt-pbuX-guaB-guaA | This work |
| S. aureus NRS384 | EK11 | ΔhsdR ΔsauUSI Δxpt-pbuX-guaB-guaA ϕ85att::pIMC85-xpt-pbuX-*guaB-guaA | This work |
| S. aureus NRS384 | EK12 | ΔhsdR ΔsauUSI Δxpt-pbuX-guaB-guaA ϕ85att::pIMC85-xpt-pbuX-guaB-*guaA | This work |
| S. aureus NRS384 | EK13 | ΔhsdR ΔsauUSI ΔguaB | This work |
| S. aureus NRS384 | EK14 | ΔhsdR ΔsauUSI Δxpt-pbuX-guaB-guaA ϕ85att::pIMC85-xpt-pbuX-guaB-guaA | This work |
| S. aureus NRS384 | EK15 | ΔhsdR ΔsauUSI ϕ85att::pIMC85-S. aureus nrdI(−218)-lacZ | This work |
| B. subtilis 1A771 | EK16 | amyE::pDG1661-S. aureus nrdI(−218)-lacZ | This work |
| K. pneumoniae subsp. pneumoniae Trevisan | ATCC 132 | ATCC | |
| E. faecalis OG1RF | ATCC 47077 | ATCC | |
| E. coli | Max Efficiency DH5α competent cells | Invitrogen | |
| S. epidermidis Evans | ATCC 14990 | ATCC |
a
Strain designations used in this study.
b
The relevant genotype is given for the S. aureus and B. subtilis strains. An asterisk indicates introduction of an engineered stop codon in the gene. For the other species, the ATCC strain or product name is given.
c
Reproduction of strain originally reported in Mandal et al. (4) and studied here.