TABLE 3.
B. anthracis strains used in this study
| Strain | Relevant characteristics | Reference |
|---|---|---|
| Ames 33 | Ames pXO1− pXO2− | 1 |
| Ames 35 | Ames pXO1+ pXO2− | 1 |
| Ames 35K | Ames 35 with pXO1K plasmid | 2 |
| Ames 35K Δ(28560–29195) | Ames 35K with GBAA_RS(28560–29195) replaced with loxP | 2 |
| Ames 35K Δ(29020–28520) | Ames 35K with GBAA_RS(29020–28520) replaced with loxP | 2 |
| Ames 35K(pL1) | Ames 35K with GBAA_RS(28725–29000) replaced with clockwise-oriented loxP | This work |
| Ames 35K(pL1L2) | Ames 35K with GBAA_RS(28725–29000) replaced with clockwise-oriented loxP and GBAA_RS(29200–28530) replaced with counterclockwise-oriented loxP | This work |
| Ames 35K(pL3) | Ames 35K with GBAA_RS(28725–29195) replaced with clockwise-oriented loxP | This work |
| Ames 35K(pL1L2F1) | Ames 35K with GBAA_RS(28725–29000) replaced with clockwise-oriented loxP, GBAA_RS(29200–28530) replaced with counterclockwise-oriented loxP, and GBAA_RS(28560–28710) replaced with FRT sequence | This work |
| Ames 35K(pL2F2) | Ames 35K with GBAA_RS(28560–29000) replaced with counterclockwise-oriented loxP and GBAA_RS(29200–28530) replaced with FRT sequence | This work |
| Ames 35K(pL5) | Ames 35K with pXO1 GBAA_RS(28725–29135) replaced with clockwise-oriented loxP | This work |
| Ames 35K(pL6) | Ames 35K with pXO1 GBAA_RS(28725–29165) replaced with clockwise-oriented loxP | This work |
| Ames 35K Δesat6 | Ames 35K with pXO1K region containing the esat6 gene replaced with PSL site | This work |