FIG 8.

Effect of Ubc9 depletion in wt cells and MxA overexpression on RABV infection. (A) U373MG cells transfected with scrambled siRNA (Sc) or siRNA targeting Ubc9-1 or Ubc9-2 were infected with RABV at an MOI of 1 for 24 h. Cell extracts were analyzed by Western blotting for Ubc9, RABV P, SUMO2/3, and actin (left), and the supernatants from cells transfected with scrambled siRNA or siRNA targeting Ubc9-1 were used for the determination of titers (right). Mean values and standard deviations from three independent experiments are shown. (B) NIH 3T3 cells transfected with the empty vector or overexpressing MxA were infected with RABV at an MOI of 1 for 24 h; cell extracts were analyzed by Western blotting for RABV P, MxA, and tubulin (left), and the supernatants were used for the determination of the titers (right). Mean values and standard deviations from three independent experiments are shown.