FIG 1.

DTMUV possesses the ability to infect a broad spectrum of mammalian cells. (A) CPE caused by DTMUV infections in various cells. The cells were infected with DTMUV at an MOI of 0.01 and examined by light microscopy at 48 h postinfection. (B) Cell viability was determined by the CellTiter 96 nonradioactive cell proliferation assay, and the percent CPE was calculated accordingly. Each assay was performed in triplicate. (C) IFA results for DTMUV-infected cells. (D) Viral growth in different cells. Viral yields in supernatants were quantitated by plaque assay on BHK-21 cells. Dotted lines, limits of detection of the plaque assay.