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. 2016 Jun 24;90(14):6489–6501. doi: 10.1128/JVI.00647-16

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Copyright © 2016 Humoud et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — SG accumulation during FCV infection. (A) CRFK cells were mock infected or infected with FCV Urbana at an MOI of 1. Infected cells, with (+) or without (−) sodium arsenite (SA) treatment, were fixed at the times indicated postinfection and stained with either mouse monoclonal antibodies specific for G3BP1 and rabbit polyclonal antiserum specific for FCV NS6/7 (A) or mouse polyclonal antibodies specific for VP1 and rabbit polyclonal antiserum specific for eIF4G (B). This was followed by staining with Alexa Fluor 488-conjugated donkey anti-rabbit IgG and Alexa Fluor 555-conjugated donkey anti-mouse IgG secondary antibodies. Nuclei were stained with To-Pro-3. Stained cells were examined by fluorescence microscopy, and representative images are shown. (C) The percentage of cells containing SGs out of the total number of cells was calculated, and the means of and standard deviations of three experimental replicates are shown. Results were analyzed by one-way ANOVA with Bonferroni corrections: ***, P < 0.001 (GraphPad Prism, version 6).