. 2016 Jun 24;90(14):6174–6186. doi: 10.1128/JVI.02877-15

TABLE 2.

Summary of the growth phenotypes of individual amino acid mutants of nonviable FMI/AAA and GKL/AAA mutants

Mutant	Nucleotide		Time of full CPE	Growth phenotype of alanine mutant^b	Variant^a	Growth phenotype of variant mutant^b
Mutant	wt	Alanine mutant^a	Time of full CPE	Growth phenotype of alanine mutant^b	Variant^a	Growth phenotype of variant mutant^b
F₂₄₄A	TTC	gcC		Nonviable
M₂₄₆A	ATG	gcc	Transfection	Viable	gTc(valine)	ts
I₂₄₈A	ATT	gcc	Transfection	qi	gTc(valine)	ts
K₂₅₉A	AAA	gcA	Transfection	cs	gcA	cs
L₂₆₀A	TTG	gca	Passage 1	qi	gTa(valine)	wt-like
G₂₅₈A	GGG	Gcc	Transfection	Viable	Gcc	wt-like

Lowercase letters indicate changed nucleotides.

ts, temperature sensitive; cs, cold sensitive; qi, quasi-infectious.