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. 2016 Jun 24;90(14):6515–6525. doi: 10.1128/JVI.00624-16

FIG 3.

FIG 3

Knockdown of AMPKα1 increases the expression of viral lytic transcripts and proteins during KSHV primary infection. (A to D) shRNA knockdown of AMPKα1 promotes the expression of KSHV lytic transcripts of RTA (A), K-bZIP (B), ORF65 (C), and to a lesser extent, latent LANA gene (D). HUVEC with knockdown of AMPKα1 were infected with KSHV for 15, 24, and 40 h and examined for the expression of KSHV lytic transcripts by RT-qPCR. (E) Knockdown of AMPKα1 increases the expression of KSHV lytic proteins RTA, K-bZIP, and ORF65 but not that of LANA. HUVEC with knockdown of AMPKα1 were infected with KSHV for 48 h and analyzed for the expression of viral proteins by Western blotting.