FIG 3.

FMDV RNA quantities shed in saliva, nasal fluid, and probang samples stratified by animal cohorts. AUCs are based on the quantities of FMDV RNA shed in different sample types. The data represent the mean of the individual AUC values within each group and its 95% CI. (A) There were no differences in FMDV RNA quantities between the three different sample types when the data for all animal cohorts were combined. (B) Vaccinated cattle shed significantly smaller quantities of FMDV RNA in nasal fluid and saliva. However, virus shedding in probang samples was higher in vaccinated cattle, but the difference was not statistically significant. (C) Persistently infected FMDV carriers shed significantly more viral RNA in probang samples than noncarriers, but there were no differences in the amounts of FMDV RNA shed in saliva and nasal fluid between carriers and noncarriers. (D and E) Four-way stratification of data illustrating a reduction in virus shedding in nasal fluid and saliva associated with vaccination and higher quantities of FMDV RNA in probang samples from FMDV carriers, regardless of vaccination status. Data are for 14 vaccinated carriers [carrier (vacc.)], 8 nonvaccinated carriers [carrier (non-vacc.)], 4 vaccinated noncarriers [non-carrier (vacc.)], and 5 nonvaccinated noncarriers [non-carrier (non-vacc.)].