FIG 1.

Construction of recombinant viruses and analysis of AcMNPV-miR-1 expression. (A) Schematic diagram of the reconstructed overexpression viruses (1×, 2×, 4×, and 8×) and the control WT and NC viruses. The gray rectangles represent the copies of the AcMNPV-miR-1 precursor, and the arrows represent the indicated genes. Recombination was conducted using the Bac-to-Bac system. (B) Relative expression of AcMNPV-miR-1 was determined by qPCR. The columns represent the fold change in AcMNPV-miR-1 expression normalized to that of 5S rRNA expression. RNA samples were isolated from infected Sf9 cells at 0, 6, 12, 24, 36, 48, and 72 h p.i., and the 2^−ΔΔCT method was used to calculate the RNA level in each sample. The quantification cycle value of the no-template control (NTC) was >40. Each reaction was performed in triplicate in at least three independent experiments. The error bars represent the standard deviations.