FIG 2.

Effects of AcMNPV-miR-1 overexpression on viral infection. (A) Relative amounts of DNA replication quantified by qPCR. The columns represent the amount of DNA normalized against the WT level. The DNA samples were derived from cells infected with NC, WT, 1×, 2×, 4×, and 8× viruses at 24 and 48 h p.i. (B) BV titer curves quantified by a TCID₅₀ endpoint dilution assay. BVs were harvested from the supernatants of the infected cells at 0, 24, 48, 72, 96, and 120 h p.i. (C) BV production in cells infected by AcMNPV-miR-1 overexpression and WT viruses. BV production was determined by qPCR. (D) The quantities of viruses embedded in ODVs were determined by qPCR. A total of 10 million purified polyhedra were used for each sample. Each reaction was performed in triplicate for three independent experiments. The error bars represent the standard deviations.