FIG 3.

Target sites of AcMNPV-miR-1 and dual-luciferase reporter assay showing the regulation of the target genes. In total, a 50 nM concentration of the AcMNPV-miR-1 mimic was used in each system; the same concentration of the Cel-miR-239b-5p mimic was used as a negative control. Luciferase reporter plasmid constructs containing the predicted binding sites of the ac18 gene (A), ac95 gene (B), and the positive-control ac94 gene (C) were cotransfected with miRNA mimics into 293 cells. A multimode reader (Tecan Infinite M200) was used to assay the luciferase activities at 48 h p.t. As a control, the activities of reporter plasmids with mutations in the ac18 gene (D), ac95 gene (E), and ac94 gene (F) were detected in the same manner. All six graphs represent the mean values of three independent experiments performed in triplicate.