FIG 2.
Depletion of AMθ does not prevent disease following intramuscular ANDV challenge. Hamsters were treated intratracheally with liposomal clodronate or control liposome on days −3 and −1 or were left untreated. On day 0, all hamsters were challenged with 80 PFU ANDV by intramuscular infection. (a) Ten days post-ANDV challenge, the number of AMθ was determined by flow cytometry by gating on FSChi/SSChi cells (as described for Fig. 1) in hamster BAL samples (**, P < 0.01; ****, P < 0.0001). (b) The depletion of AMθ did not prevent disease in hamsters. Lung tissue specimens isolated from all hamsters 10 days postchallenge were evaluated for viral genome (c) by real-time (RT)-PCR (not significant).