FIG 2.

Regulation by CD151 siRNA was efficient and specific. (A) CD151 protein levels were analyzed by Western blot analysis of EA.hy926 cells 48 h after transfection with CD151 or nontargeting (NT) siRNA. A nonspecific band produced by the CD151 antibody served as a loading control. (B) Live EA.hy926 cells were immunostained for CD151 using a Cy3-conjugated secondary antibody (red) after siRNA treatment (NT or CD151 siRNA). After fixation, nuclei were counterstained with DAPI (blue). (C) The indicated siRNAs were transiently cotransfected into EA.hy926 cells with a plasmid conferring immediate early antigen (IE-Ag) expression in the absence of infection. Error bars show standard errors of the means. n.s., not significant; *, significant (P < 0.05; Student's t test).