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. 2016 Jul 7;7:966. doi: 10.3389/fpls.2016.00966
Stage Steps Procedure to obtain it Runtime
A 1–24 Fixation and embedding in paraffin 8 days
B Undetermined Microscopy: sectioning and microscope pre-selection ~5 h per mold
C 25–47 Paraffin removal and hybridization with LNA double-labeled probes 1 day
D 48–62 Detection and development 1 day
E 63–65 Mounting and photographing ~30 min per slide