Figure 3.

Quantification of iNOS expression. Human liver HC04 cells were mock infected or infected with B. pseudomallei or its rpoS mutant or E. coli B at MOI 10 and at 2, 4 and 8 h.p.i. RNA was extracted and expression of iNOS determined by qRT-PCR. The level of iNOS expression was normalized against the expression of β-actin. Statistical analyses were undertaken using SigmaPlot 11.0 one-way ANOVA. Lower case letters indicate a significant difference from the corresponding upper case letters (P-value <0.05). Lower case letters with a star indicate that the rpoS mutant was statistically different from wild type.