Fig. 3.

GSK-3 differentially regulates 4EBP1 and S6RP phosphorylation in ACHN cells. a Changes in the phosphorylation levels of 4EBP1 and S6RP over time after treatment of AR-A014418 (25 μM) or rapamycin (100 nM) to ACHN cells. b ACHN cells were treated with LY294002 (10 μM) for the indicated times. In a and b, the cells were lysed and then analyzed for the phosphorylation of 4EBP1 and S6RP by immunoblotting. Data are representative of at least three independent immunoblot experiments