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. 2016 Jul 7;16:393. doi: 10.1186/s12885-016-2418-7

Fig. 4.

Fig. 4

In vitro kinase assays. In vitro kinase assays were performed using recombinant GSK-3β and recombinant 4EBP1 as described in the Materials and methods. Either AR-A014418 (25 μM) or SB-216763 (25 μM) was applied in vitro to inhibit recombinant GSK-3β. p4EBP1 levels were measured with immunoblot analysis. Data are representative of three separate immunoblot analysis