FIG 5.

S. oralis and H₂O₂ mediate lysosomal destruction. RAW 264 cells were exposed to the S. oralis WT or H₂O₂ for 3 h and cultured for an additional 3 and 21 h (total, 6 and 24 h, respectively) in fresh medium containing antibiotics. At the indicated times after exposure, nuclei and LAMP-1 were fluorescently labeled with DAPI and Alexa Fluor 488-conjugated anti-LAMP-1 monoclonal antibody, respectively. Bar = 10 μm.