FIG 8.

Effect of deferoxamine on lysosomal damage in S. oralis-infected macrophages. RAW 264 cells were treated with 0.5 or 2 mM deferoxamine and then infected with viable S. oralis strains for 3 h. The cells were washed with PBS and cultured in fresh medium containing antibiotics for 21 h. (A) Viability was determined by trypan blue staining. *, P < 0.05 compared with the untreated control (None). (B) The cells were stained with LysoTracker after 3 h of infection (top) and were also immunostained using LAMP-1 antibodies after 6 h of infection (bottom). Bar = 10 μm.