FIG 4.

Positive effect of rpiRc inactivation on transcript levels of sarA. Northern blot analysis of sar transcripts from S. aureus strain SA564, the rpiRc deletion mutant (SA-rpiRc), and the rpiRc mutant carrying the cis-integrated plasmid pEC4-rpiRc for complementation (SA-rpiRc_rpiRc) cultivated for the indicated times was performed. The sar locus originates from three different promoters (P1, P3, and P2) resulting in transcripts that differ in size (0.56, 0.8, and 1.2 kb, respectively). The blot is representative of at least two independent experiments.