FIG 1.

Toxoplasma gondii strain-dependent inhibition of luminescence in IFN-γ-inducible FLUC reporter lines. A tandem GAS element-driven FLUC reporter was stably expressed in HeLa cells (A) or RAW264.7 macrophages (B). Reporter cell lines were plated in 96-well plates and infected with different MOIs, as indicated. At 2 h after infection, the cells were stimulated with 100 U of IFN-γ/ml for 18 h. Subsequently, the cells were lysed, and the luciferase activity was measured. The data shown are means ± standard errors of the mean (SEM) of at least three independent experiments, each with three technical replicates. Kruskal-Wallis test with Dunn's correction (***, P < 0.0001) was performed for GT1 and each of the other T. gondii strains.