Skip to main content
. 2016 Jun 23;84(7):1994–2001. doi: 10.1128/IAI.00077-16

FIG 2.

FIG 2

Mapping of a single target locus on chromosome XII. (A) GT1 (type I) and ME49 (type II) parental strains and 36 progeny were tested for their ability to interfere with the GAS-FLUC in RAW264.7 macrophages. The data shown are normalized averages of three technical replicates ± standard deviations (SD). (B and C) QTL scan of normalized percent inhibition identified a single locus of ∼688 kb (TGME49_chrXII: 6134501.0.6822749) on chromosome XII defined by the two markers 181 and 193 and the progeny SF50 and SF34. Fine mapping with additional PCR markers (see Table S2 in the supplemental material) within the QTL identified two additional informative progeny (2C7C3 and SF59) and narrowed the region responsible for the observed strain difference to ∼263 kb (TGME49_chrXII: 6559896.0.6822749) comprising 36 annotated genes.