Subject area |
Biology |
More specific subject area |
Immunohistochemistry |
Type of data |
Figure |
How data was acquired |
Microscope, LC-MS/MS |
Data format |
Analysed |
Experimental factors |
Aorta root cryosections (8μm thickness) with the presence of aorta valve cups were prepared by freezing microtome. |
Experimental features |
For immunohistochemistry analysis, aorta roots were dealt with using specific antibodies against TNF-α, ICAM-1, VCAM-1 and MOMA-2, respectively; For NANA analysis, cryosections were subjected to ligation with FTSC; For LC-MS/MS analysis, NANA was released from aorta by acid hydrolysis and then quantified by LC-MS/MS with precursor to product ion transition of m/z 308.1→86.7. |
Data source location |
Institute of atherosclerosis, Taishan Medical University, Tai’an, China. |
Data accessibility |
Data are presented in this article. |