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. 2016 Jun 22;8:613–617. doi: 10.1016/j.dib.2016.06.020
Subject area Biology
More specific subject area Immunohistochemistry
Type of data Figure
How data was acquired Microscope, LC-MS/MS
Data format Analysed
Experimental factors Aorta root cryosections (8μm thickness) with the presence of aorta valve cups were prepared by freezing microtome.
Experimental features For immunohistochemistry analysis, aorta roots were dealt with using specific antibodies against TNF-α, ICAM-1, VCAM-1 and MOMA-2, respectively; For NANA analysis, cryosections were subjected to ligation with FTSC; For LC-MS/MS analysis, NANA was released from aorta by acid hydrolysis and then quantified by LC-MS/MS with precursor to product ion transition of m/z 308.1→86.7.
Data source location Institute of atherosclerosis, Taishan Medical University, Tai’an, China.
Data accessibility Data are presented in this article.