Figure 1.

AtPIP1;4 affects plant immunity and cytoplasmic H₂O₂ accumulation. A, Northern-blot hybridization with probes specific for AtPIP1;4 and for the reference gene ACTIN2. B, Logarithmic colony formation units (cfu) of bacteria recovered from plant leaves. Six replicates were used; error bars indicate se. Different letters indicate significant differences in a multiple comparison (P < 0.01). C, Leaves at 7 dpi and necrosis severity (mean ± se; n = 6). D, Western blotting of the leaf PM fraction hybridized with antibodies against GFP and the PM marker protein H⁺-ATPase. E, Imaging of AUR- or AR-stained leaves and H₂O₂ content in leaf cells (mean ± se; n = 3). r.u., Relative units; WT, wild type.