Figure 2.

De novo AtPIP1;4 expression mediates H₂O₂ translocation in yeast. A, AtPIP1;4 probe hybridization to the blot of total RNA from yeast cells transformed with the empty vector and the AtPIP1;4-containing vector. B and C, Yeast H₂O₂ content and viability after 45 min of H₂O₂ treatment (mean ± se). Asterisks indicate significant differences in paired comparisons (n = 6; P < 0.01). D, AUR and AR probing of yeast cells 45 min after treatment with 0 or 3 mm H₂O₂. E to G, Chronological changes in the H₂O₂ content in yeast cells after treatment with 0 or 3 mm H₂O₂ (mean ± se; n = 3).